Coding

Part:BBa_K3286283:Experience

Designed by: Santiago Castanedo Fontanillas   Group: iGEM19_Wageningen_UR   (2019-10-21)


This part was transformed into E. coli and the lysate were run in a SDS-PAGE gel. Then, the gel was transferred to a Western blot membrane and incubated with anti 6x-His tag antibody. After visualizing the membrane, two clear bands were observable, corresponding to the truncated and the untruncated versions of the protein. This proves that this part works. From the membrane, it was also clear that the frequency in which the truncated protein was expressed was lower than that of the untruncated one.

Figure 1:Differential expression of the truncated and untruncated proteins, when using a ribosomal frameshifting sequence in a fusion protein.

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